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p416cyc vector  (New England Biolabs)


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    New England Biolabs p416cyc vector
    P416cyc Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p416cyc+vector/AgeI-HF/pmc04344410__NIHMS647096___supplement___supplement_1-50-12-21
    Average 97 stars, based on 877 article reviews
    p416cyc vector - by Bioz Stars, 2026-09
    97/100 stars

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    Amplification:

    Article Title: Massively Parallel Single Amino Acid Mutagenesis
    Article Snippet: .. The resulting tag cassette amplicon was directionally 119 cloned into the modified p416CYC vector by double-digestion with AgeI-HF and MfeI-HF 120 (NEB) and transformed into ElectroMax DH10B electrocompetent E. coli (Invitrogen), to yield 121 ~9.2x10 6 distinctly tagged clones. .. The resulting library, p416CYC-bc, was expanded by bulk 122 outgrowth and purified by midiprep using the ChargeSwitch Pro Midi kit (Invitrogen).

    Clone Assay:

    Article Title: Massively Parallel Single Amino Acid Mutagenesis
    Article Snippet: .. The resulting tag cassette amplicon was directionally 119 cloned into the modified p416CYC vector by double-digestion with AgeI-HF and MfeI-HF 120 (NEB) and transformed into ElectroMax DH10B electrocompetent E. coli (Invitrogen), to yield 121 ~9.2x10 6 distinctly tagged clones. .. The resulting library, p416CYC-bc, was expanded by bulk 122 outgrowth and purified by midiprep using the ChargeSwitch Pro Midi kit (Invitrogen).

    Modification:

    Article Title: Massively Parallel Single Amino Acid Mutagenesis
    Article Snippet: .. The resulting tag cassette amplicon was directionally 119 cloned into the modified p416CYC vector by double-digestion with AgeI-HF and MfeI-HF 120 (NEB) and transformed into ElectroMax DH10B electrocompetent E. coli (Invitrogen), to yield 121 ~9.2x10 6 distinctly tagged clones. .. The resulting library, p416CYC-bc, was expanded by bulk 122 outgrowth and purified by midiprep using the ChargeSwitch Pro Midi kit (Invitrogen).

    Plasmid Preparation:

    Article Title: Massively Parallel Single Amino Acid Mutagenesis
    Article Snippet: .. The resulting tag cassette amplicon was directionally 119 cloned into the modified p416CYC vector by double-digestion with AgeI-HF and MfeI-HF 120 (NEB) and transformed into ElectroMax DH10B electrocompetent E. coli (Invitrogen), to yield 121 ~9.2x10 6 distinctly tagged clones. .. The resulting library, p416CYC-bc, was expanded by bulk 122 outgrowth and purified by midiprep using the ChargeSwitch Pro Midi kit (Invitrogen).

    Transformation Assay:

    Article Title: Massively Parallel Single Amino Acid Mutagenesis
    Article Snippet: .. The resulting tag cassette amplicon was directionally 119 cloned into the modified p416CYC vector by double-digestion with AgeI-HF and MfeI-HF 120 (NEB) and transformed into ElectroMax DH10B electrocompetent E. coli (Invitrogen), to yield 121 ~9.2x10 6 distinctly tagged clones. .. The resulting library, p416CYC-bc, was expanded by bulk 122 outgrowth and purified by midiprep using the ChargeSwitch Pro Midi kit (Invitrogen).



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